69077 Sigma-AldrichpET-30 Ek/LIC Vector Kit - Novagen
Novagen's pET-30 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the His-Tag and S-Tag coding sequences that are cleavable with enterokinase protease.
More>> Novagen's pET-30 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the His-Tag and S-Tag coding sequences that are cleavable with enterokinase protease. Less<<pET-30 Ek/LIC Vector Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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Products
| Catalogue Number | Packaging | Qty/Pack | |
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| 69077-3CN | Plastic ampoule | 20 rxn |
| Description | |
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| Overview | The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product. |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 69077 |
| Brand Family | Novagen® |
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| Quality Level | MQ100 |
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| Specifications |
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| Catalogue Number | GTIN |
| 69077-3CN | 07790788055134 |
Documentation
pET-30 Ek/LIC Vector Kit - Novagen Certificates of Analysis
| Title | Lot Number |
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| 69077 |
Brochure
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| Radiance! Multisystem Protein Expression |
Citations
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User Protocols
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| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB055 pET System Manual |
| TB163 Ek/LIC Cloning Kits |
| TB184 Xa/LIC Cloning Kits |
Vector Map
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| TB162VM pET-30 Ek/LIC Vector Map |
Vector Sequence
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| pET-30 Ek/LIC Vector Sequence |