71147 Sigma-AldrichpACYCDuet™-1 DNA - Novagen
pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site.
More>> pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site. Less<<pACYCDuet™-1 DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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Products
| Catalogue Number | Packaging | Qty/Pack | |
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| 71147-3CN | Glass bottle | 10 μg |
| Description | |
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| Overview | Coexpression of multiple target genes in E. coli is advantageous for studying protein complexes. Coexpression often achieves optimal yield, solubility, and activity and may protect individual subunits from degradation. The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. The Duet vectors
carry compatible replicons and antibiotic resistance markers and may be used together in appropriate host strains to coexpress up to eight proteins. Certain combinations of Duet vectors and pET or pETcoco™ vectors are also compatible for coexpression. The capability of Duet vectors to be cotransformed, propagated, and induced for robust target protein
coexpression makes them ideal for the analysis of protein complexes. The Duet vectors are designed with compatible replicons and drug resistance genes for effective propagation and maintenance of four plasmids in a single cell. pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be transformed into the same cell with pETDuet™-1, pCDFDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 in the appropriate host strain for the coexpression of up to eight target genes. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 71147 |
| Brand Family | Novagen® |
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| Product Information | |
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| Fusion tag | His•Tag, S•Tag |
| Vector family | Duet |
| Quality Level | MQ100 |
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| Ship Code | Shipped with Blue Ice or with Dry Ice |
| Toxicity | Standard Handling |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71147-3CN | 04055977254808 |
Documentation
pACYCDuet™-1 DNA - Novagen SDS
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pACYCDuet™-1 DNA - Novagen Certificates of Analysis
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| 71147 |
Citations
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User Protocols
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| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB340 Duet Vectors |
Vector Map
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| TB336VM pACYCDuet™-1 Vector Map |
Vector Sequence
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| pACYCDuet-1 Vector Sequence |