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114582 mEC-broth with Novobiocin

114582
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

ph ValueSolubility
6.7 - 7.1 (36.7 g/l, H₂O, 25 °C) (after autoclaving)36.7 g/l

Products

Catalogue NumberPackaging Qty/Pack
1.14582.0500 Plastic bottle 500 g
Description
Catalogue Number114582
References
Product Information
HS Code3821 00 00
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
pH value6.7 - 7.1 (36.7 g/l, H₂O, 25 °C) (after autoclaving)
Solubility36.7 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Appearance (clearness)clear
Appearance (color)yellowish to yellowish-braun
pH-value (25 °C)6.7 - 7.1
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157))≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090))≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))≥ 10000
Inoculum on reference medium (Proteus hauseri ATCC 13315)≥ 10000
Growth (Escherichia coli ATCC 35150 (O157))> 10 transparent colonies with a pale yellowish-brown appearance on SMAC agar
Growth (Escherichia coli ATCC 11775 (WDCM 00090))> 10 pink colonies on SMAC Agar
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))total inhibition on TSA
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))total inhibition on TSA
Mixed inoculum of E.coli, P.hauseri and E.faecalis
Additionaly monoculture of E.faecalis
Confirmation on SMAC agar resp. Tryptic soy agar
Incubation:
mEC broth: 18 - 24 hours at 41,5 ± 1 °C aerobic
Confirmation on SMAC agar resp. Tryptic Soy Agar: 18 - 24 hours at 35 ± 1 °C aerobic
Global Trade Item Number
Catalogue Number GTIN
1.14582.0500 04022536558554

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Categories

Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Microbiological Water Testing for Hospital & Clinical
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Coliforms, E. coli & Enterobacteriaceae
Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Microbiological Water Testing for Pharmaceutical & Cosmetics Industry
Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Microbiological Water Testing for Food & Beverage Industry > Enrichment
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Product > Dehydrated and Ready-to-use Culture Media
Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Water Testing for Municipal & Environmental Water
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > E. Coli O157 & STEC