Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

WB59 Western-Re-Probe™ Reagent

This Western-Re-Probe™ Reagent is validated for use in Immunoblotting.

Western-Re-Probe™ Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
WB59
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
WB59-100MLCN Plastic ampoule 100 ml
Description
OverviewDesigned to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
A special formulation designed to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity.
Catalogue NumberWB59
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationSupplied as a 5X solution.
Kit contains5X Re-Probe solution (125 ml)
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (see comments)
Application CommentsSufficient volume to strip 25-30 standard blots.

ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes

PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.

Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetection
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains5X Re-Probe solution (125 ml)
Specifications
Global Trade Item Number
Catalogue Number GTIN
WB59-100MLCN 04055977206746

Documentation

Western-Re-Probe™ Reagent SDS

Title

Safety Data Sheet (SDS) 

Western-Re-Probe™ Reagent Certificates of Analysis

TitleLot Number
WB59
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-July-2009 RFH
ApplicationImmunoblotting (see comments)
DescriptionWestern-Re-Probe™ Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.
FormLiquid
FormulationSupplied as a 5X solution.
CommentsSufficient volume to strip 25-30 standard blots.

ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes

PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.

Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetection
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling