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03-232 Ubiquitin activating enzyme E1 (His-tag) human recombinant

Human recombinant Ubiquitin activating enzyme E1 (His-tag).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays UniProt Number: P22314
03-232
50 µg  
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Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesUni Prot Number
Enzyme AssaysHuman P22314
Description
Catalogue Number03-232
Brand Family Upstate
Trade Name
  • Upstate
DescriptionUbiquitin activating enzyme E1 (His-tag) human recombinant
OverviewHuman recombinant Ubiquitin activating enzyme E1 (His-tag)
Background InformationUbiquitin activating enzyme E1 is responsible for the first step in the formation of the ubiquitin-protein isopeptide bond. It is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating, with ATP, the C-terminal glycine carboxyl group of ubiquitin and thenlinking this residue to the sulphydryl side chain moiety of a cysteine residue in E1, by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
References
Product Information
FormatPurified
PresentationIn liquid containing 50 mM Tris-HCl, pH 7.5, containing 1.0 mM DTT. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant Ubiquitin activating enzyme E1 (His-tag).
Key Applications
  • Enzyme Assays
Biological Information
HostE. coli
SourceFull length human ubiquitin-activating enzyme E1 containing a C-terminal His-tag
UniProt Number
Molecular Weight~119 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-232 04053252550928

Documentation

Ubiquitin activating enzyme E1 (His-tag) human recombinant SDS

Title

Safety Data Sheet (SDS) 

Ubiquitin activating enzyme E1 (His-tag) human recombinant Certificates of Analysis

TitleLot Number
Ubiquitin activating enzyme E1 (His-tag) human recombinant 3071737
Ubiquitin activating enzyme E1 (His-tag) human recombinant - 3011125 3011125
Ubiquitin activating enzyme E1 (His-tag) human recombinant - 4066267 4066267
Ubiquitin activating enzyme E1 (His-tag) human recombinant - Q2022485 Q2022485
Ubiquitin activating enzyme E1 (His-tag) human recombinant -2573070 2573070
Ubiquitin activating enzyme E1 (Histag) human recombinant - VP1308216 VP1308216