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662065 Ubiquitin, Methylated, Human, Recombinant

Ubiquitin, Methylated, Human, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Me-Ub

662065
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
662065-1MGCN Plastic ampoule 1 mg
Description
OverviewRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
Catalogue Number662065
Brand Family Calbiochem®
SynonymsMe-Ub
References
ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
Product Information
FormLyophilized solid
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
662065-1MGCN 04055977261127

Documentation

Ubiquitin, Methylated, Human, Recombinant SDS

Title

Safety Data Sheet (SDS) 

Ubiquitin, Methylated, Human, Recombinant Certificates of Analysis

TitleLot Number
662065

References

Reference overview
Hershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsMe-Ub
DescriptionRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
FormLyophilized solid
Recommended reaction conditionsTypical concentrations for non-rate-limiting support of in vitro conjugation reactions range from 200 µM to 1 mM depending on the reaction conditions.
Purity≥95% by SDS-PAGE
SolubilityAqueous buffers (50 mg/ml)
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.