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70624 Tuner™(DE3)pLysS Competent Cells - Novagen

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.

Tuner™(DE3)pLysS Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70624
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70624-3CN Plastic ampoule 0.4 ml
70624-4CN Glass bottle 1 ml
Description
OverviewTuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F– ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) pLysS (CamR)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70624
Brand Family Novagen®
References
Product Information
Components
Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/µg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70624-3CN 04055977257519
70624-4CN 04055977257526

Documentation

Tuner™(DE3)pLysS Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

Tuner™(DE3)pLysS Competent Cells - Novagen Certificates of Analysis

TitleLot Number
70624

Brochure

Title
Competent Cell Brochure

Citations

Title
  • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
  • Daniel W. Schuerch, Elizabeth M. Wilson-Kubalek and Rodney K. Tweten. (2005) Molecular basis of listeriolysin O pH dependence. Proceedings of the National Academy of Sciences (USA) 102, 12537-12542.
  • Simon R. Andrews, et al. (2004) The use of forced protein evolution to investigate and improve stability of family 10 Xylanases: the production of Ca2+-independent stable xylanases. Journal of Biological Chemistry 279, 54369-54379.
  • M. Schuster, M. L. Urbanowski and E. P. Greenberg. (2004) Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR. Procedings of the National Academy of Science 101, 15833-15839.
  • Rongkun Shen, et al. (2004) Escherichia coli nucleoside diphosphate kinase interactions with T4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. Journal of Biological Chemistry 279, 32225-32232.
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    User Protocols

    Title
    TB009 Competent Cells
    TB053 Academic and Non-profit Laboratory Assurance Letter