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650279 Trypsin, Porcine, MS Grade

Trypsin, Porcine, MS Grade MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
650279
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
650279-1SETCN Glass bottle 1 set
Description
OverviewA chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer.
Catalogue Number650279
Brand Family Calbiochem®
References
ReferencesHenzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316..
Product Information
Unit of DefinitionOne BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥10,000 U/mg
Physicochemical Information
ContaminantsChymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
S PhraseS: 22-24-26-36/37-45

Do not breathe dust.
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Harmful
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
650279-1SETCN 04055977185225

Documentation

Trypsin, Porcine, MS Grade SDS

Title

Safety Data Sheet (SDS) 

Trypsin, Porcine, MS Grade Certificates of Analysis

TitleLot Number
650279

References

Reference overview
Henzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316..
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-May-2009 JSW
DescriptionA chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry.
FormLyophilized
Recommended reaction conditions
Materials Provided: • Trypsin, Porcine, MS Grade (Kit Component No. KP31816-20UG): 5 vials, 20 µg each • Trypsin Resuspension Buffer (Kit Component No. KP31854-0.5ML): 1 vial, 0.5 ml Preparation Before Use: • Before opening the trypsin centrifuge the tube at 15,000 x g for 5 min. Suspend the MS Grade Trypsin in the Trypsin-Suspension Buffer. Recommended reconstitution concentration 0.5-1 µg/µl. IMPORTANT NOTE: In some cases, after reconstitution, there is insoluble material, which is a non-protein and will not interfere with trypsin activity. If you prefer to remove it, centrifuge the tube briefly and transfer the supernatant to a clean tube. Guidelines for use: • For mass spectrometry sequence analysis, MS Grade Trypsin to protein ratio of 1:20 to 1:100 is recommended. • Use freshly reconstituted MS Grade Trypsin in any sequence analysis. Any unused trypsin may be stored at -70°C and used within two weeks. Avoid repeated freeze-thaw cycle after re-suspension, which may lead to performance loss. • Any available protocol can be used for digestion of proteins with MS Grade Porcine Trypsin.
ContaminantsChymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Specific activity≥10,000 U/mg
Unit definitionOne BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Toxicity Harmful
ReferencesHenzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316..