Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

612200 Tissue Plasminogen Activator, Human

Tissue Plasminogen Activator, Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: tPA

612200
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
612200-100UGCN Plastic ampoule 100 μg
612200-1MGCN Glass bottle 1 mg
Description
OverviewNative tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acids. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration.
Catalogue Number612200
Brand Family Calbiochem®
SynonymstPA
References
ReferencesKim, Y.H., et al. 1999. Science 284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol. 19, S21.
Product Information
FormLyophilized
FormulationLyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween®-80 Detergent.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
SourcePrepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥300 KU/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
612200-100UGCN 04055977187762
612200-1MGCN 04055977187755

Documentation

Tissue Plasminogen Activator, Human SDS

Title

Safety Data Sheet (SDS) 

Tissue Plasminogen Activator, Human Certificates of Analysis

TitleLot Number
612200

References

Reference overview
Kim, Y.H., et al. 1999. Science 284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol. 19, S21.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-May-2011 JSW
SynonymstPA
DescriptionNative tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca2+ antagonist may increase levels of tPA.
FormLyophilized
FormulationLyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween®-80 Detergent.
SourcePrepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Purity≥95% by SDS-PAGE
Specific activity≥300 KU/mg protein
SolubilityReconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesKim, Y.H., et al. 1999. Science 284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol. 19, S21.