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565835 SHIP1 Inhibitor, 3AC - Calbiochem

SHIP1 Inhibitor, 3AC, CAS 2206-20-4, is a cell-permeable, selective inhibitor of SHIP1 polyphosphatase activity toward PIP3 (IC50 = 10 µM), but has no effect on SHIP2 5′ or PTEN activity on PIP3.

SHIP1 Inhibitor, 3AC - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3α-Aminocholestane, SH2-domain-containing inositol 5ʹ-phosphatase 1 (SHIP1) Inhibitor, 3AC

565835
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₇H₄₉N

Products

Catalogue NumberPackaging Qty/Pack
565835-10MGCN Glass bottle 10 mg
Description
OverviewA cell-permeable steroidal compound that selectively inhibits SHIP1′ polyphosphatase activity toward PI(3,4,5)P3 (IC50 = 10 µM), but not SHIP2 5′ or PTEN′ polyphosphatase activity toward PI(3,4,5)P3 (IC50 > 1 mM). Effectively inhibits SHIP1-mediated immune response both in vitro and in vivo, as well as SHIP1-dependent cancer cell survival. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
Catalogue Number565835
Brand Family Calbiochem®
Synonyms3α-Aminocholestane, SH2-domain-containing inositol 5ʹ-phosphatase 1 (SHIP1) Inhibitor, 3AC
References
ReferencesBrooks, R., et al. 2010. J. Immunol. 184, 3582.
Product Information
FormWhite solid
Hill FormulaC₂₇H₄₉N
Chemical formulaC₂₇H₄₉N
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationSHIP1 Inhibitor, 3AC, CAS 2206-20-4, is a cell-permeable, selective inhibitor of SHIP1 polyphosphatase activity toward PIP3 (IC50 = 10 µM), but has no effect on SHIP2 5′ or PTEN activity on PIP3.
Biological Information
Purity≥98% by 1H NMR
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
565835-10MGCN 04055977191929

Documentation

SHIP1 Inhibitor, 3AC - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

SHIP1 Inhibitor, 3AC - Calbiochem Certificates of Analysis

TitleLot Number
565835

References

Reference overview
Brooks, R., et al. 2010. J. Immunol. 184, 3582.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2012 JSW
Synonyms3α-Aminocholestane, SH2-domain-containing inositol 5ʹ-phosphatase 1 (SHIP1) Inhibitor, 3AC
DescriptionA cell-permeable steroidal compound that selectively inhibits SHIP1-, but not SHIP2-, catalyzed PI(3,4,5)P3-to-PI(3,4)P2 dephosphorylation (IC50 = 10 µM vs. >1 mM, respectively), while exhibiting little activity against the PTEN-catalyzed PI(3,4,5)P3-to-PI(4,5)P2 conversion (IC50 > 1 mM). 3AC in vivo treatment (2.292 umol/200 µl/mouse/daily i.p. for 7 d) is reported to boost both peripheral blood granulocyte production (by 4- to 5-fold) and the GVHD- (graft-versus-host disease) suppressing Mac1+Gr1+ MIR (myeloid immunoregulatory) cell population in lymphoid tissues (by 5.55- and 11.53-fold in spleen and lymph nodes, respectively), resulting in an impaired splenocyte allogeneic T cell response in MLR (mixed-leukocyte/lymphocyte reaction) assays (by ≥95%). Similarly, 3AC-treated (9.4 µM; 24 h) primary human PBMCs greatly lose their ability in priming allogeneic T cell response in MLR assays (by 74.5%). In addition, a faster blood cell recovery is also observed in mice receiving 3AC (2.292 umol/200 µl/mouse/daily i.p. for 7 d) after sub-lethal irradiation (550 Rads). Selectively inhibits the growth and survival of SHIP1-dependent human KG-1 and murine C1498 myelogenous leukemia lines (IC50 = 11.5 µM), but not the SHIP1-negative human CML K562 and osteosarcoma MG63 lines. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₇H₄₉N
Structure formulaStructure formula
Purity≥98% by 1H NMR
SolubilityEthanol (100 mg/ml). Not soluble in DMSO.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesBrooks, R., et al. 2010. J. Immunol. 184, 3582.