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552095 R-Spondin 4, Mouse, Recombinant

R-Spondin 4, Mouse, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Cristin 4

552095
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
552095-25UGCN Glass bottle 25 μg
Description
OverviewRecombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
Catalogue Number552095
Brand Family Calbiochem®
SynonymsCristin 4
References
ReferencesHendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
552095-25UGCN 04055977268294

Documentation

R-Spondin 4, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

R-Spondin 4, Mouse, Recombinant Certificates of Analysis

TitleLot Number
552095

References

Reference overview
Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2011 RFH
SynonymsCristin 4
DescriptionRecombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
SolubilityReconstitute to 200 µg/ml in PBS.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.