ECM509 Sigma-AldrichQCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with fluorescent detection.
More>> The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with fluorescent detection. Less<<MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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Key Spec Table
| Key Applications | Detection Methods |
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| ACT | Fluorescent |
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| Catalogue Number | ECM509 |
| Brand Family | Chemicon® |
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| Description | QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric |
| Overview | Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here Introduction Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable. The CHEMICON® QCM™ 24-well Cell Migration Assay does not require cell labeling, scraping, washing or counting. The 24-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. In the CHEMICON® QCM™ 24-well Migration Assay, invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQUANTâ GR dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). The CHEMICON® QCM™ 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells. The CHEMICON® QCM™ 24-well Migration Assay utilizes an 8 mm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including: · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510) · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512) · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515) · QCM™ 96-well Cell Invasion Assay (ECM555) · QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556) · 24-well Insert Cell Migration and Invasion Assay Systems · CytoMatrix™ Cell Adhesion strips (ECM protein coated) · QuantiMatrix™ ECM protein ELISA kits Test Principle The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membrane, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis. |
| Materials Required but Not Delivered | 1. Precision pipettes: sufficient for aliquoting cells. 2. Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators. Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery. 3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS. 4. Chemoattractants (eg. 10% FBS) or pharmacological agents for addition to culture medium, if screening is desired. 5. Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA. Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer. 6. Sterile PBS or HBSS to wash cells. 7. Distilled water. 8. Low speed centrifuge and tubes for cell harvesting. 9. CO2 incubator appropriate for subject cells. 10. Hemocytometer or other means of counting cells. 11. Trypan blue or equivalent viability stain. 12. Fluorescence plate reader. 13. Sterile cell culture hood. |
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| Detection method | Fluorescent |
| Quality Level | MQ100 |
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| Storage Conditions | Store kit materials at 2-8°C for up to their expiration date. Do not freeze. |
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| Material Size | 1 plate |
| Material Package | 24 wells |
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| Catalogue Number | GTIN |
| ECM509 | 04053252668852 |
Documentation
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric SDS
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References
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| RNAi-mediated silencing of vEGF-C inhibits non-small cell lung cancer progression by simultaneously down-regulating the cXCR4, cCR7, vEGFR-2 and vEGFR-3-dependent axes-induced ERK, p38 and AKT signalling pathways. Feng Y, Hu J, Ma J, Feng K, Zhang X, Yang S, Wang W, Zhang J, Zhang Y European journal of cancer (Oxford, England : 1990) 2011 Show Abstract | 21680174
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Brochure
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| Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression |
| Cell Migration and Invasion: Choosing the Right Assay |
| EpiGRO™, EndoGRO™ and FibroGRO™ Reagents |
Data Sheet
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| Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation |
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Categories
| Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays |