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539480 Proteinase K, Tritirachium album

Proteinase K, Tritirachium album MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
539480
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
539480-100MGCN Plastic ampoule 100 mg
539480-25MGCN Plastic ampoule 25 mg
Description
OverviewSerine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Catalogue Number539480
Brand Family Calbiochem®
References
ReferencesWatazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.
Product Information
CAS number39450-01-6
Activity≥30 mAnsonU/mg dry weight
Unit of DefinitionOne mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 37°C, pH 7.5.
EC number3.4.21.14
FormWhite lyophilized solid
FormulationLyophilized from calcium acetate
Quality LevelMQ300
Applications
Biological Information
Specific Activity≥40 mAnsonU/mg protein
Physicochemical Information
ContaminantsDNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539480-100MGCN 04055977269147
539480-25MGCN 07790788051327

Documentation

Proteinase K, Tritirachium album SDS

Title

Safety Data Sheet (SDS) 

Proteinase K, Tritirachium album Certificates of Analysis

TitleLot Number
539480

References

Reference overview
Watazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.

Brochure

Title
Bulk Product Guide
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-November-2016 JSW
DescriptionSerine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
FormWhite lyophilized solid
FormulationLyophilized from calcium acetate
Recommended reaction conditions

Anson Enzyme Activity Assay: Materials Required • 5 ml of Hemoglobin (20 mg/ml) in 100 mM phosphate buffer, pH 7.5 (6 M urea, 5 mM NaCl). • 0.6 ml of 20 mM CaCl2 dissolved in distilled water. • 0.1 ml of proteinase K (40 µg/ml) in 20 mM CaCl2 Activity Assay Protocol 1. Mix the 5 ml hemoglobin, 0.6 ml CaCl2, and 0.1 ml proteinase K and make up the volume to 6 ml with 20 mM CaCl2; incubate for 10 min at 25°C. 2. Add 10 ml 300 mM Trichloroacetic acid and incubate at room temperature for 30 min. 3. Centrifuge and transfer 5 ml of the supernatant to a clean tube. Add 10 ml 500 mM NaOH and 3 ml Folin reagent (diluted 1:2 with redistilled water); incubate for 5 min at room temperature. 4. Measure the ΔA at 750 nm. For calibration use tyrosine solutions. 1 mAnson unit liberates 1 µmole of Folin-positive amino acids per min (calculated as tyrosine) under test conditions.
CAS number39450-01-6
EC number3.4.21.14
ContaminantsDNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
Specific activity≥40 mAnsonU/mg protein
Activity≥30 mAnsonU/mg dry weight
Unit definitionOne mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 37°C, pH 7.5.
Solubility50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.
Toxicity Irritant
ReferencesWatazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.