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14-573M Pim1 Protein, active, 250 µg

Active, N-terminal GST-tagged recombinant human full length Pim-1, for use in Kinase Assays.

Pim1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-573M
250 µg  Also available in 10 μg size (cat#14-573) and in bulk (cat# 14-573-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-573M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPim1 Protein, active, 250 µg
OverviewN-terminal GST-tagged recombinant human full length Pim-1
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST-tagged recombinant human full length Pim-1, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM]
Gene Symbol
  • PIM1
  • PIM
Protein TargetPim-1
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P11309 # Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.
COFACTOR: Manganese.
SIZE: 404 amino acids; 45412 Da
SUBUNIT: Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Nucleus. & Isoform 1: Cell membrane.
TISSUE SPECIFICITY: Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
PTM: Autophosphorylated on both serine/threonine and tyrosine residues.
SIMILARITY: SwissProt: P11309 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily. & Contains 1 protein kinase domain.
Molecular Weight62kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of S6 Kinase/Rsk-2 Substrate Peptide 2
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-573) and in bulk (cat# 14-573-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-573M 04053252742484

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-573 Pim1 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases