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70748 Pellet Paint® NF Co-Precipitant

Non-fluorescent visible DNA co-precipitant for automated sequencing applications

Pellet Paint® NF Co-Precipitant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70748
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70748-3CN Plastic ampoule 125 rxn
70748-4CN Plastic ampoule 1000 rxn
Description
Overview

Pellet Paint NF Co-Precipitant is a nonfluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with 1 µl of carrier per reaction and centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labeled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Resuspension of pelleted sequencing reaction products in deionized formamide can be confirmed by checking for dissolution of the carrier. Pellet Paint NF Co-Precipitant is fully compatible with the ABI PRISM® BigDye Terminator Cycle Sequencing Ready Reaction. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer before the cycle sequencing reaction. Although Pellet Paint NF absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet Paint NF) can be used as a correction factor when determining nucleic acid concentration. Pellet Paint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequence accuracy. Pellet Paint NF Co-Precipitant is a useful substitute for the original Pellet Paint Co-Precipitant in applications where fluorescent detection is used.

Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending
Catalogue Number70748
Brand Family Novagen®
Features and benefits
  • Efficient and rapid precipitation of BigDye® cycle sequencing products
  • Efficient removal of dye terminators
  • Direct visualization and tracking of precipitated material
  • No effect on sequencing reaction
  • Substitute for original Pellet Paint® Co-Precipitant for fluorescent detection applications
References
Product Information
•125 µl or 1 mlPellet Paint NF Co-Precipitant
•1 ml or 8 ml3 M Sodium Acetate, pH 5.2
DeclarationThis product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates.
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70748-3CN 04055977273090
70748-4CN 07790788052812

Documentation

Pellet Paint® NF Co-Precipitant SDS

Title

Safety Data Sheet (SDS) 

Pellet Paint® NF Co-Precipitant Certificates of Analysis

TitleLot Number
70748

Brochure

Title
Bulk Product Guide
High fidelity gene amplification

Citations

Title
  • Taku Nakagawa, et al. (2007) Detection of circulating tumor cells in early-stage breast cancer metastasis to axillary lymph nodes. Clinical Cancer Research 13, 4105-4110.
  • Hikaru Taira, Takahiro Hohsaka and Masahiko Sisido. (2006) In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system. Nucleic Acids Research 34, 1653-1662.
  • Youn-Soo Lee, et al. (2005) Coexpression of erythropoietin and erythropoietin receptor in von Hippel-Lindau disease-associated renal cysts and renal cell carcinoma. Clinical Cancer Research 11, 1059-1064.
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    User Protocols

    Title
    TB268 Pellet Paint® NF Co-Precipitant