Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

109272 Papanicolaou's solution 3b polychromatic solution EA 50

for cytology

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109272
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1.09272.0500 Plastic bottle 500 ml
1.09272.1000 Plastic bottle 1 l
1.09272.2500 Plastic bottle 2.5 l
Description
Catalogue Number109272
DescriptionPapanicolaou's solution 3b polychromatic solution EA 50
OverviewPapanicolaou’s solution 3b polychromatic solution EA 50 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65(Cat.no. 109270), o Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Papanicolaou’s solution 3b polychromatic solution EA 50 gives a blue-green-pinkish staining result in clinical specimen material.
The 500 ml bottle provides 1500 - 2000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor cytology
Biological Information
Physicochemical Information
Density0.82 g/cm3 (20 °C)
Flash point17 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H302 + H312 + H332: Harmful if swallowed, in contact with skin or if inhaled.
H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
H370: Causes damage to organs.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P312: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor if you feel unwell.
P308 + P311: IF exposed or concerned: Call a POISON CENTER/doctor.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal1
Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of dangerhighly flammable, toxic
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1987 , 3, II
Declaration (transport by air) IATA-DGRUN 1987 , 3, II
Declaration (transport by sea) IMDG-CodeUN 1987 , 3, II
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)passes test
Nucleiblue to dark violet
Cyanophilic Cytoplasm (basophilic)blue-green
Eosinophilic Cytoplasm (acidophilic)pink
Global Trade Item Number
Catalogue Number GTIN
1.09272.0500 04022536112886
1.09272.1000 04022536676685
1.09272.2500 04022536112909

Documentation

Papanicolaou's solution 3b polychromatic solution EA 50 SDS

Title

Safety Data Sheet (SDS) 

Papanicolaou's solution 3b polychromatic solution EA 50 Certificates of Analysis

Product Number PackagingSpecificationLot Number
1.09272.0500 Plastic bottlePDF Specification Document
1.09272.1000 Plastic bottlePDF Specification Document
1.09272.2500 Plastic bottlePDF Specification Document

User Guides

Title
User Guide (IFU) - 109272

Related Products & Applications

Accessories (Primary)

Catalogue Number Description
109270 Papanicolaou's solution 3c Polychromatic solution EA 65
106887 Papanicolaou’s solution 2b Orange II solution
106888 Papanicolaou's solution 2a Orange G solution (OG 6)
109253 Papanicolaou's solution 1a Harris' hematoxylin solution
109254 Papanicolaou's solution 1b Hematoxylin solution S
109269 Papanicolaou's solution 3d polychromatic solution EA 65
109271 Papanicolaou's solution 3a polychromatic solution EA 31

Accessories (Secondary)

Catalogue Number Description
109270 Papanicolaou's solution 3c Polychromatic solution EA 65
106887 Papanicolaou’s solution 2b Orange II solution
106888 Papanicolaou's solution 2a Orange G solution (OG 6)
109253 Papanicolaou's solution 1a Harris' hematoxylin solution
109254 Papanicolaou's solution 1b Hematoxylin solution S
109269 Papanicolaou's solution 3d polychromatic solution EA 65
109271 Papanicolaou's solution 3a polychromatic solution EA 31

Related Products By: Application Facete

Clinical Cytology
Cytodiagnosis
Papanicolaou

Categories

IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents