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14-335M PRAK Protein, unactive, 250 µg

Unactive, recombinant full-length human PRAK with an N-terminal His-tag, for use in Kinase Assays.

14-335M
250 µg  Also available in 50 mg size (cat#14-335)
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-335M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPRAK Protein, unactive, 250 µg
OverviewRecombinant full-length human PRAK with an N-terminal His-tag
References
Product Information
Quality LevelMQ300
Applications
ApplicationUnactive, recombinant full-length human PRAK with an N-terminal His-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf9 cells
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
Gene Symbol
  • MAPKAPK5
  • PRAK
Protein TargetPRAK
Purification MethodNi-NTA agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q8IW41 # Mediates stress-induced small heat shock protein 27 phosphorylation.
SIZE: 473 amino acids; 54220 Da
SUBUNIT: Interacts with SQSTM1.
SUBCELLULAR LOCATION: Cytoplasm (Probable). Nucleus. Note=Also observed in the nucleus.
TISSUE SPECIFICITY: Expressed ubiquitously.
PTM: Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
SIMILARITY: SwissProt: Q8IW41 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight54kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of PRAK Substrate Peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -20C
Packaging Information
Material Size250 µg
Material PackageAlso available in 50 mg size (cat#14-335)
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-335M 04053252924156

Documentation

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-335 PRAK Protein, unactive, 50 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases