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539568 PP2B, Human, Recombinant, E. coli

PP2B, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CaN, Protein Phosphatase 2B, Calcineurin

539568
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
539568-5000UCN Plastic ampoule 5000 u
Description
OverviewRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
Catalogue Number539568
Brand Family Calbiochem®
SynonymsCaN, Protein Phosphatase 2B, Calcineurin
References
ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.
Product Information
Activity≥50,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; <a href ="/Products/ProductDisplay.asp→catNO=207008">Cat. No. 207008</a>) in the presence of calmodulin per min at 30°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
Quality LevelMQ100
Applications
Biological Information
PurityTwo distinct bands by SDS-PAGE
Specific Activity≥300,000 units/mg
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539568-5000UCN 04055977194685

Documentation

PP2B, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

PP2B, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
539568

References

Reference overview
Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2011 JSW
SynonymsCaN, Protein Phosphatase 2B, Calcineurin
DescriptionRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
PurityTwo distinct bands by SDS-PAGE
Specific activity≥300,000 units/mg
Activity≥50,000 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
Toxicity Standard Handling
ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.