Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

33-036 PI 3-Kinase HTRF™ Assay; 10,000 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.

PI 3-Kinase HTRF™ Assay; 10,000 wells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
33-036
10,000 wells  Kit capacity: 10,000 wells
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number33-036
Brand Family Upstate
Trade Name
  • HTRF
  • Upstate
DescriptionPI 3-Kinase HTRF™ Assay; 10,000 wells
References
Product Information
Components
  • 4X Reaction Buffer
  • PIP2 (Substrate; 1mM stock)
  • Stop Reagent A
  • Stop Reagent B
  • DM A Detection Reagent
  • DM B Detection Reagent
  • DM C Detection Reagent
Detection methodHTRF
Quality LevelMQ100
Applications
ApplicationThe PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.
Key Applications
  • Kinase Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • PI 3-Kinase
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • PIK3CA
  • MGC142163
  • PI3K
  • MGC142161
  • p110-alpha
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions12 months at -70°C
Packaging Information
Material Size10,000 wells
Material PackageKit capacity: 10,000 wells
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
33-036 04053252621345

Documentation

PI 3-Kinase HTRF™ Assay; 10,000 wells SDS

Title

Safety Data Sheet (SDS) 

PI 3-Kinase HTRF™ Assay; 10,000 wells Certificates of Analysis

TitleLot Number
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2055085 2055085
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2071624 2071624
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2071639 2071639
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2071640 2071640
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2110954 2110954
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2110956 2110956
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2138157 2138157
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2138158 2138158
PI3 Kinase 4-Step Assay Reagent Kit (10000 wells) - 2201841 2201841

Related Products & Applications

Alternative Packsize

Catalogue Number Description
33-016 PI 3-Kinase HTRF™ Assay; 384 wells
33-017 PI 3-Kinase HTRF™ Assay; 1920 wells
33-037 PI 3-Kinase HTRF™ Assay; 50,000 wells

Alternative Format

Catalogue Number Description
33-047 PI 3-Kinase 3-step HTRF™ Assay

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Related Products By: Brand Facete

HTRF™

Categories

Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Enzyme Assay Kits and Reagents