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69284 NovaBlue(DE3) Competent Cells - Novagen

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, DE3 lysogen cannot be used for the blue/white screening of recombinant plasmids.

NovaBlue(DE3) Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69284
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69284-3CN Plastic ampoule 0.4 ml
69284-4CN Glass bottle 1 ml
Description
OverviewNovaBlue(DE3) Competent Cells is a K-12 strain with high transformation efficiency and recA endA mutations. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacIq repressor encoded by the F episome. 

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number69284
Brand Family Novagen®
References
Product Information
Components
Guaranteed efficiencyGuaranteed efficiency: > 1 x 10⁸ cfu/μg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69284-3CN 04055977259377
69284-4CN 04055977259384

Documentation

NovaBlue(DE3) Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

NovaBlue(DE3) Competent Cells - Novagen Certificates of Analysis

TitleLot Number
69284

Citations

Title
  • Mark S. McClain and Timothy L. Cover. (2007) Functional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin. Infection and Immunity 75, 1785-1793.
  • Yuichi Hongoh, et al. (2006) Phylogenetic diversity, localization, and cell morphologies of members of the candidate phylum TG3 and a subphylum in the phylum Fibrobacteres, recently discovered bacterial groups dominant in termite guts. Applied and Enviornmental Microbiology 72, 6780-6788.
  • Ryan Simkovsky and Jonathan King. (2006) An elongated spine of buried core residues necessary for in vivo folding of the parallel β-helix of P22 tailspike adhesin. Proceedings of the National Academy of Sciences (USA) 103, 3575-3580.
  • Steven D. Breazeale, et al. (2005) A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function of UDP-4-deoxy-4-formamido-L-arabinose. Journal of Biological Chemistry 280, 14154-14167.
  • Dale K. Shumaker, et al. (2005) Functions and dysfunctions of the nuclear lamin Ig-fold domain in nuclear assembly, growth, and Emery-Dreifuss muscular dystrophy. Proceedings of the National Academy of Sciences (USA) 102, 15494-15499.
  • John V. McDowell, et al. (2004) Demonstration of the involvement of outer surface protein E coiled coil structural domains and higher order structural elements in the binding of infection-induced antibody and the complement-regulatory protein, factor H. Journal of Immunology 173, 7471-7480.
  • An X. Tran, et al. (2004) Periplasmic cleavage and modification of the 1-phosphate group of Helicobacter pylori lipid A. Journal of Biological Chemistry 279, 55780-55791.
  • Jae Hyung Koo, Ick Hyun Cho and Yu Sam Kim. (2000) The malonate decarboxylase operon of Acinetobacter calcoaceticus KCCM 40902 is regulated by malonate and the transcriptional repressor MdcY. Journal of Bacteriology 182, 6382-6390.
  • C. Hatfield, et al. (1997) Epitope mapping by recombination PCR mutagenesis. Bio/Techniques 22, 332-337.
  • K.D. Janda, et al. (1997) Selection for catalysis in combinatorial antibody libraries. Science 275, 945-948.
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    User Protocols

    Title
    TB009 Competent Cells
    TB053 Academic and Non-profit Laboratory Assurance Letter