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AC146 Normal Chicken IgY Antibody, purified | AC146

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Research Sub-Category: Isotype Control Antibodies
AC146
100 µg  
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Description
Catalogue NumberAC146
Trade Name
  • Chemicon
DescriptionNormal Chicken IgY Antibody, purified | AC146
Background InformationChickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week's eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.
Product Information
FormatPurified
PresentationPurified chicken polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
Key Applications
  • Blocking of Intefering Antibodies
Application NotesGel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain.

Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491.

Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.
Biological Information
Concentration1 mg/mL
HostChicken
IsotypeIgY
Antibody TypePolyclonal Antibody
Purification MethodT-gel
Molecular Weight~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting using non-reduced Chicken IgY.

Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
AC146 04053252944727