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480450 NFF-2 - Calbiochem

A quenched fluorescence substrate for MMPs.

NFF-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MCA-RPKPYANvaWMK(DNP)-NH₂

480450
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
480450-1MGCN Plastic ampoule 1 mg
Description
OverviewA quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 5.9 x 104 M-1sec-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 5 x 104 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 5.5 x 104 M-1s-1).
Supplied as a trifluoroacetate salt.
Catalogue Number480450
Brand Family Calbiochem®
SynonymsMCA-RPKPYANvaWMK(DNP)-NH₂
References
ReferencesNagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
Product Information
ATP CompetitiveN
FormLyophilized yellow solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₇₉H₁₀₅N₁₉O₁₉S
Chemical formulaC₇₉H₁₀₅N₁₉O₁₉S
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetMatrix Metalloproteinases (MMPs)
Primary Target IC<sub>50</sub>kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceMCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
480450-1MGCN 04055977201376

Documentation

NFF-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

NFF-2 - Calbiochem Certificates of Analysis

TitleLot Number
480450

References

Reference overview
Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-August-2008 RFH
SynonymsMCA-RPKPYANvaWMK(DNP)-NH₂
DescriptionA quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 59,400 M-1s-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 54,000 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 55,300 M-1s-1).
FormLyophilized yellow solid
FormulationSupplied as a trifluoroacetate salt.
Chemical formulaC₇₉H₁₀₅N₁₉O₁₉S
Peptide SequenceMCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)
Purity≥98% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from moisture
Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesNagase, H., et al. 1994. J. Biol. Chem. 269, 20952.