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475954 MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem

The MRN-ATM Pathway Inhibitor, Mirin, also referenced under CAS 299953-00-7, controls the biological activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex. This small molecule/inhibitor is primarily used for Cell Structure applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin

475954
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
299953-00-7C₁₀H₈N₂O₂S

Products

Catalogue NumberPackaging Qty/Pack
475954-10MGCN Plastic ampoule 10 mg
Description
OverviewA cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
Catalogue Number475954
Brand Family Calbiochem®
Synonyms(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
References
ReferencesGarner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.
Product Information
CAS number299953-00-7
FormReddish orange powder
Hill FormulaC₁₀H₈N₂O₂S
Chemical formulaC₁₀H₈N₂O₂S
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
475954-10MGCN 04055977184297

Documentation

MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem Certificates of Analysis

TitleLot Number
475954

References

Reference overview
Garner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-August-2009 RFH
Synonyms(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
DescriptionA cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
FormReddish orange powder
Intert gas (Yes/No) Packaged under inert gas
CAS number299953-00-7
Chemical formulaC₁₀H₈N₂O₂S
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (10 mg/ml) or Ethanol (3 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesGarner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.