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14-638M MLCK Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays.

MLCK Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-638M
250 µg  Also available in 10 μg size (cat#14-638) and in bulk (cat# 14-638-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-638M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMLCK Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
Gene Symbol
  • MYLK
  • DKFZp686I10125
  • MLCK108
  • FLJ12216
  • MSTP083
  • KRP
  • MLCK210
  • MLCK
  • Telokin
Protein TargetMLCK
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15746 # Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
COFACTOR: Magnesium. & Calcium.
SIZE: 1914 amino acids; 210774 Da
SUBUNIT: All isoforms including Telokin bind calmodulin.
TISSUE SPECIFICITY: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
PTM: MLCK is probably down-regulated by phosphorylation.
SIMILARITY: SwissProt: Q15746 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 fibronectin type-III domain. & Contains 9 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight43.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of ZIPtide (Catalog # 12-545)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-638) and in bulk (cat# 14-638-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-638M 04053252403828

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-638 MLCK Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases