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48-610MAG MILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Signaling, Apoptosis & Cancer
48-610MAG
96-well plate  96-well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
STAT1 (Tyr701) STAT2 (Tyr690) STAT3 (Tyr705) STAT5A/B (Tyr694/699) STAT6 (Tyr641) HMplexLuminex xMAP
Description
Catalogue Number48-610MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay
OverviewThe STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • STAT1 (Tyr701)
  • STAT2 (Tyr690)
  • STAT3 (Tyr705)
  • STAT5A/B (Tyr694/699)
  • STAT6 (Tyr641)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
48-610MAG 04053252599385

Documentation

MILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX®MAP Cell Signaling and Cytokine Panels
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
MILLIPLEX®MAP Cell Signaling Wall Chart