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HCYTA-60K-PX48 MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A 48 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay

The Human Cytokine/Chemokine/Growth Factor Panel A bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 48 multiple cytokine, chemokine, and growth factor biomarkers in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms, Inflammation, Infectious Diseases, Cytokines & Cytokine Receptors, Growth Factors & Receptors, Oncology, Obesity, Metabolic Disorders
HCYTA-60K-PX48
96-well plate  96 well plate
Purchase on Sigma-Aldrich

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Overview

Replacement Information

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
sCD40L EGF Eotaxin FGF-2 Flt-3 ligand Fractalkine G-CSF GM-CSF GROα IFNα2 IFNγ IL-1α IL-1β IL-1ra IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8 IL-9 IL-10 IL-12 (p40) IL-12 (p70) IL-13 IL-15 IL-17A IL-17E/IL-25 IL-17F IL-18 IL-22 IL-27 IP-10 MCP-1 MCP-3 M-CSF MDC (CCL22) MIG MIP-1α MIP-1β PDGF-AA PDGF-AB/BB RANTES TGFα TNFα TNFβ VEGF-A HMplexLuminex xMAP
Description
Catalogue NumberHCYTA-60K-PX48
ReplacesHCYTMAG-60K-PX38 and HCYTMAG-60K-PX41
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A 48 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay
OverviewMILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses.“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Growth factors are involved in the stimulation of target cell survival, proliferation, differentiation with effects on angiogenesis, vasculogenesis, cell migration, apoptosis, wound healing and embryogenesis.

Cytokine, chemokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process, and disease states such as infectious disease, osteoarthritis, respiratory disease, IBD, sepsis, allergic reactions as well as neurologic, metabolic and cardiovascular disease and even cancer.
References
Product Information
Detection methodLuminex xMAP
HS Code3002 15 90
ConfigurationPremixed
Precision, %
  • Intra-assay: <15%
  • Inter-assay: <20%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationThe Human Cytokine/Chemokine/Growth Factor Panel A bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 48 multiple cytokine, chemokine, and growth factor biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of neat plasma or serum (1:100 dilution for RANTES) or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all of the Human Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
• RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
• This premixed panel comes with a RANTES bead, so please plan your samples accordingly.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • sCD40L
  • EGF
  • Eotaxin
  • FGF-2
  • Flt-3 ligand
  • Fractalkine
  • G-CSF
  • GM-CSF
  • GROα
  • IFNα2
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-1ra
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-9
  • IL-10
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17E/IL-25
  • IL-17F
  • IL-18
  • IL-22
  • IL-27
  • IP-10
  • MCP-1
  • MCP-3
  • M-CSF
  • MDC (CCL22)
  • MIG
  • MIP-1α
  • MIP-1β
  • PDGF-AA
  • PDGF-AB/BB
  • RANTES
  • TGFα
  • TNFα
  • TNFβ
  • VEGF-A
Cross ReactivityThere was no or negligible cross-reactivity observed between the antibodies for any single analyte and any of the other analytes in this panel, except IL-12 (p40). IL-12 (p40) exhibited a cross-reactivity of approximately 8% with IL-12 (p70). This is not unexpected since the p40 subunit is a constituent of IL-12 (p70).
Accuracy
  • 92% - 106%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
Standard Curve Range
  • MIP-1β: 0.38 - 6,000 pg/mL
  • IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-18, MDC: 0.64 - 10,000 pg/mL
  • FLT-3L: 0.96 - 15,000 pg/mL
  • GROα, IFNγ, IL-3, IL-17A, RANTES, TGFα: 1.3 - 20,000 pg/mL
  • IL-1β, IL-1RA, TNFβ: 1.6 - 25,000 pg/mL
  • GM-CSF, IL-10, IP-10, VEGF-A: 2.6 - 40,000 pg/mL
  • EGF, Eotaxin, IL-12 (p70), IL-15, MCP-1, MIP-1α: 3 - 50,000 pg/mL
  • G-CSF, IL-1α: 4.8 - 75,000 pg/mL
  • IL-12 (p40), IL-13, MIG, TNFα: 6.4 - 100,000 pg/mL
  • IFNα2, MCP-3: 8 - 125,000 pg/mL
  • PDGF-AB/BB: 9.6 - 150,000 pg/mL
  • sCD40L, IL-22, PDGF-AA: 13 - 200,000 pg/mL
  • IL-27: 16 - 250,000 pg/mL
  • FGF-2: 26 - 400,000 pg/mL
  • Fractalkine, IL-17F: 32 - 500,000 pg/mL
  • IL-17E/IL-25, M-CSF: 40 - 625,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HCYTA-60K-PX48 04061841058601