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426100 Lactacystin, Synthetic - CAS 1258004-00-0 - Calbiochem

Lactacystin, Synthetic, CAS 1258004-00-0, is an irreversible inhibitor of 20S proteasome inhibitor (IC₅₀ = 500 nM). A covalent inhibitor of the chymotrypsin & trypsin-like activities of proteasome.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Proteasome Inhibitor VI

Primary Target: 20S proteasome (MCP; multicatalytic proteinase complex)
426100
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
1258004-00-0C₁₅H₂₄N₂O₇S

Products

Catalogue NumberPackaging Qty/Pack
426100-1MGCN Glass bottle 1 mg
426100-200UGCN Plastic ampoule 200 μg
426100-400UGCN Plastic ampoule 400 μg
Description
OverviewAn irreversible proteasome inhibitor. A Streptomyces metabolite that acts as a highly specific inhibitor of the 20S proteasome (MCP; multicatalytic proteinase complex) (IC50 = 500 nM for 20S proteasome). Blocks proteasome activity by targeting the catalytic β-subunit. Acts as a covalent inhibitor of the chymotrypsin- and trypsin-like activities of the proteasome. Induces neurite outgrowth in Neuro 2A mouse neuroblastoma cells and inhibits progression of synchronized Neuro 2A cells and MG-63 human osteosarcoma cells beyond the G1 phase of the cell cycle. Reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 µM).
Catalogue Number426100
Brand Family Calbiochem®
SynonymsProteasome Inhibitor VI
References
ReferencesKeyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem. 31, 279.
Dick, L.R., et al. 1996. J. Biol. Chem. 271, 7273.
Oda, K., et al. 1996. Biochem. Biophys. Res. Commun. 219, 800.
Fenteany, G., et al. 1995. Science 268, 726.
Imajoh-Ohmi, S., et al. 1995. Biochem. Biophys. Res. Commun. 217, 1070.
Jensen, T.J., et al. 1995. Cell 83, 129.
Katagiri, M.M., et al. 1995. J. Antibiot. 48, 344.
Mori, S., et al. 1995. J. Biol. Chem. 270, 29447.
Tanaka, H., et al. 1995. Biochem. Biophys. Res. Commun. 216, 291.
Fenteany, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3358.
Omura, S., et al. 1991. J. Antibiot. 44, 117.
Product Information
CAS number1258004-00-0
ATP CompetitiveN
FormWhite solid
Hill FormulaC₁₅H₂₄N₂O₇S
Chemical formulaC₁₅H₂₄N₂O₇S
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Target20S proteasome (MCP; multicatalytic proteinase complex)
Primary Target IC<sub>50</sub>500 nM against 20S proteasome; 10 µM against NF-κB activation
Purity≥99% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution in DMSO, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Unstable in aqueous solutions; reconstitute in H₂O just prior to use.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
426100-1MGCN 04055977187311
426100-200UGCN 04055977187328
426100-400UGCN 04055977187335

Documentation

Lactacystin, Synthetic - CAS 1258004-00-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Lactacystin, Synthetic - CAS 1258004-00-0 - Calbiochem Certificates of Analysis

TitleLot Number
426100

References

Reference overview
Keyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem. 31, 279.
Dick, L.R., et al. 1996. J. Biol. Chem. 271, 7273.
Oda, K., et al. 1996. Biochem. Biophys. Res. Commun. 219, 800.
Fenteany, G., et al. 1995. Science 268, 726.
Imajoh-Ohmi, S., et al. 1995. Biochem. Biophys. Res. Commun. 217, 1070.
Jensen, T.J., et al. 1995. Cell 83, 129.
Katagiri, M.M., et al. 1995. J. Antibiot. 48, 344.
Mori, S., et al. 1995. J. Biol. Chem. 270, 29447.
Tanaka, H., et al. 1995. Biochem. Biophys. Res. Commun. 216, 291.
Fenteany, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3358.
Omura, S., et al. 1991. J. Antibiot. 44, 117.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Proteasomes Technical Bulletin

Citations

Title
  • Keyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
  • Lacher, M.D., et al. 2006. Cancer Research 66, 1648.
  • Wu, C-J., et al. 2005. European Molecular Biology Organization Journal 24, 1886.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-October-2012 JSW
    SynonymsProteasome Inhibitor VI
    DescriptionAn irreversible proteasome inhibitor. A Streptomyces metabolite that acts as a highly specific inhibitor of the 20S proteasome (MCP; multicatalytic proteinase complex) (IC50 = 500 nM for 20S proteasome). Blocks proteasome activity by targeting the catalytic β-subunit. Acts as a covalent inhibitor of the chymotrypsin and trypsin-like activities of the proteasome. Induces neurite outgrowth in Neuro 2A mouse neuroblastoma cells beyond theG1 phase of the cell cycle. Reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 µM)
    FormWhite solid
    CAS number1258004-00-0
    Chemical formulaC₁₅H₂₄N₂O₇S
    Structure formulaStructure formula
    Purity≥99% by HPLC
    SolubilityDMSO (50 mg/ml) or H₂O
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution in DMSO, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Unstable in aqueous solutions; reconstitute in H₂O just prior to use.
    Toxicity Standard Handling
    ReferencesKeyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
    Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem. 31, 279.
    Dick, L.R., et al. 1996. J. Biol. Chem. 271, 7273.
    Oda, K., et al. 1996. Biochem. Biophys. Res. Commun. 219, 800.
    Fenteany, G., et al. 1995. Science 268, 726.
    Imajoh-Ohmi, S., et al. 1995. Biochem. Biophys. Res. Commun. 217, 1070.
    Jensen, T.J., et al. 1995. Cell 83, 129.
    Katagiri, M.M., et al. 1995. J. Antibiot. 48, 344.
    Mori, S., et al. 1995. J. Biol. Chem. 270, 29447.
    Tanaka, H., et al. 1995. Biochem. Biophys. Res. Commun. 216, 291.
    Fenteany, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3358.
    Omura, S., et al. 1991. J. Antibiot. 44, 117.
    Citation
  • Keyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
  • Lacher, M.D., et al. 2006. Cancer Research 66, 1648.
  • Wu, C-J., et al. 2005. European Molecular Biology Organization Journal 24, 1886.
    		•