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391947 Hydroxylapatite, Fast Flow

Hydroxylapatite, Fast Flow MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HA

391947
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
391947-100GMCN Glass bottle 100 gm
Description
OverviewA form of calcium phosphate used in preparative procedures for purification of proteins and nucleic acids in which high flow rate and binding capacity are desired. BSA binding capacity: >12 mg/g; DNA binding capacity: >700 µg/g.
Catalogue Number391947
Brand Family Calbiochem®
SynonymsHA
References
Product Information
CAS number1306-06-5
FormWhite solid
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
391947-100GMCN 07790788049843

Documentation

Hydroxylapatite, Fast Flow SDS

Title

Safety Data Sheet (SDS) 

Hydroxylapatite, Fast Flow Certificates of Analysis

TitleLot Number
391947
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-May-2008 RFH
SynonymsHA
DescriptionA form of calcium phosphate used in preparative procedures for purification of proteins and nucleic acids in which high flow rate and binding capacity are desired (BSA binding capacity: >12 mg/g; DNA binding capacity: >700 µg/g).
FormWhite solid
Recommended reaction conditions
Hydroxylapatite, Fast Flow Protocol Re-hydration: Determine the amount of hydroxylapatite necessary for the intended application. Re-hydrate with 4-6 volumes of starting buffer, such as low ionic strength phosphate buffer, pH >5.5. Gently swirl to thoroughly re-suspend the crystals. Allow the slurry to settle. Decant the excess buffer to approximately give an equal volume of buffer to settled hydroxylapatite. Gently resuspend for a second time in an equal volume of buffer before use. Packing the Column: Close the outlet and half-fill the column with starting buffer. Pour the desired amount of fully suspended hydroxylapatite through an attached filling funnel and allow the suspension to settle. After the hydroxylapatite has stabilized, open the column and pour at lease two bed volumes of starting buffer through the column at the appropriate hydrostatic pressure. Prior to the addition of sample, conductivity, pH, or absorbance of the effluent can be used to confirm column equilibration. Sample Application and Elution: In general, samples are loaded on the hydroxylapatite in low ionic strength phosphate buffers and are eluted with stepwise or gradient concentration increases. The integrity of the column packing and an accurate measure of the void volume can be determined by applying a small amount of low molecular weight sample which does not bind to hydroxylapatite (e.g. 0.01% methyl orange). This will provide a visual check of uniformity as it passes through the column. Regeneration of Hydroxylapatite: High ionic strength phosphate buffer (400-500 mM) is normally sufficient to desorb most material from hydroxylapatite. The use of a high salt solution (1-2 M NaCl), followed by extensive re-equilibration, may help in the removal of contaminants. If necessary, the contaminated top portion of a hydroxylapatite column may be removed, and the column repacked before subsequent use.
CAS number1306-06-5
SolubilityPhosphate buffer
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C).
Toxicity Standard Handling
Merck USA index14, 3467