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SCR544 Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
SCR544
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCR544
Trade Name
  • STEMCCA
DescriptionHuman STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit
OverviewThe Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The STEMCCA vector is comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.
References
Product Information
Components
  • 1. Human STEMCCA Constitutive (OKSM) Lentivirus: (Part number SCR544-1) Two (2) vials of EF1α-hSTEMCCA (OKSM) Lentivirus (Part number CS204502). Each vial contains 15 µL of high titer lentivirus.
  • 2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.
HS Code3002 15 90
Quality LevelMQ100
Applications
ApplicationThe Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.
Biological Information
Stem Cell Type
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceTested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsLentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR544 04053252013805

Documentation

Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
Human Stem Cell Systems
Simplifying the iPSC Workflow
Stem Cell Research

Technical Info

Title
Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Data Sheet

Title
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
STEMCCA Lentivirus Reprogramming Kits

User Guides

Title
Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit