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EZMCP1-99K5PKRM Human MCP-1 Conferma™ ELISA (5 Pack Bulk)

This Human MCP-1 Conferma™ ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

EZMCP1-99K5PKRM
96-well strip plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberEZMCP1-99K5PKRM
DescriptionHuman MCP-1 Conferma™ ELISA (5 Pack Bulk)
Background InformationHuman MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. In particilar this is a marker of inflammatory disorders. This sandwich ELISA uses our Conferma™ process to ensure a high degree of lot to lot consistency.
References
Product Information
Detection methodColorimetric
Precision, %
  • Inter-assay: <12.9% CV
  • Intra-assay: <4.9% CV
Quality LevelMQ200
Applications
ApplicationThis Human MCP-1 Conferma™ ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.
Key Applications
  • ELISA
Application Notes3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)
Biological Information
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Analytes Available
  • MCP-1
Entrez Gene Number
Entrez Gene Summary This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4
Gene Symbol
  • HC11
  • MCAF
  • MCP1
  • MCP-1
  • SCYA2
  • GDCF-2
  • SMC-CF
  • HSMCR30
UniProt Number
UniProt SummaryFUNCTION: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
SIZE: 212 amino acids; 23,718 Da
SUBUNIT: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
SUBCELLULAR LOCATION: Extracellular region or secreted
DISEASE: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
SIMILARITY: Belongs to the IL-6 superfamily.
Accuracy
  • 84-95%
Physicochemical Information
Linearity of DilutionSerum Linearity: 94-106% Serum Parallelism: 91-115% Plasma Linearity: 94-110% Plasma Parallelism: 95-114%
Standard Curve Range
  • 1.17 pg/mL - 300 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EZMCP1-99K5PKRM 04061841718567

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