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SCR603 Human ES/iPS Neurogenesis Kit

The human ES/iPS Neurogenesis Kit is a robust and user-friendly kit that contains the necessary serum free/chemically defined media and reagents for the rapid production of expandable neural progenitor cells and terminally differentiated neurons from pluripotent human ES/iPS cells

Human ES/iPS Neurogenesis Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Neuroscience, Cell Differentiation
SCR603
1/Kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Neuroscience, Diff
Description
Catalogue NumberSCR603
DescriptionHuman ES/iPS Neurogenesis Kit
Alternate Names
  • NIM
  • NDM
  • NEM
Background InformationEMD Millipore’s Human ES/iPS Neurogenesis Kit is a robust and user-friendly kit that contains the necessary serum free/chemically defined media and reagents for the rapid production of expandable neural progenitor cells and terminally differentiated neurons from pluripotent human ES and iPS cells. The media contained in this kit rely on both small molecules neural inducers and supplements to generate a highly enriched population of expandable neural progenitors and end stage neurons based on establish protocols (1,2). Neural progenitors are generated 10 days from starting cultures of traditional feeder-based and/or feeder-free cultures of undifferentiated human ES/iPS cells and can be expanded for over 3-5 passages resulting in a minimum 20-fold expansion. Typically, from 1 well of a 6-well plate containing an estimated 500,000 pluripotent human ES/iPS, approximately 5-10 million neural progenitors can be obtained after 3 passages. The resulting neural progenitor cells are multipotent and can be further differentiated to specific neural cell subtypes of interest.
References
Product Information
Components
  • 1) Human ES/iPS Neural Induction Medium (Cat. No. SCM110): 125 mL bottle containing optimized medium for the directed induction of human pluripotent cells to expandable neural progenitor cells. Sufficient reagents are provided for six reactions in a 6 well plate format.
  • 2) ENStem-A Neural Expansion Medium (Cat. No. SCM004): 500 mL bottle containing optimized medium and growth factors for expanding neural progenitor cells.
  • 3) Human ES/iPS Neuronal Differentiation Medium (Cat. No. SCM111): 100 mL bottle containing optimized medium & reagents for the rapid differentiation of neural progenitors to terminal neurons. Sufficient reagents are provided to differentiate 24 reactions in a 24 well format for 2 weeks.
Quality LevelMQ100
Applications
ApplicationThe human ES/iPS Neurogenesis Kit is a robust and user-friendly kit that contains the necessary serum free/chemically defined media and reagents for the rapid production of expandable neural progenitor cells and terminally differentiated neurons from pluripotent human ES/iPS cells
Key Applications
  • Neuroscience
  • Cell Differentiation
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20C. Good for 3 months from date of receipt or until expiration date on bottle when reagents are handled and stored appropriately.
Packaging Information
Material Size1/Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR603 04053252855405