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376816 Hexokinase II VDAC Binding Domain Peptide, Cell-Permeable - Calbiochem

A cell-permeable peptide analog of Hexokinase II VDAC binding domain peptide.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HXK2VBD-cpm, H-RQIKIWFQNRRMKWKK-MIASHLLAYFFTELN-NH₂

Primary Target: Detach and translocate HXK2 from mitochondria to the cytosol
376816
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₈₈H₂₉₁N₅₃O₄₀S₂

Products

Catalogue NumberPackaging Qty/Pack
376816-1MGCN Plastic ampoule 1 mg
Description
OverviewA cell-permeable peptide analog of Hexokinase II VDAC binding domain peptide (Cat. No. 376815). The internalization domain of the Antennapedia homeoprotein is fused to the methionine amino terminal. Shown to completely detach and translocate HXK2 from mitochondria to the cytosol in HeLa cells at 100 µM. Does not induce Bax translocation or cytochrome c release when used alone. However, it markedly sensitizes cells to cytochrome c release and to the induction of apoptosis when used in combination with a Bax-dependent apoptosis inducer, Indomethacin (Cat. No. 405268).
Catalogue Number376816
Brand Family Calbiochem®
SynonymsHXK2VBD-cpm, H-RQIKIWFQNRRMKWKK-MIASHLLAYFFTELN-NH₂
References
ReferencesPastorino, J.G., et al. 2002. J. Biol. Chem. 277, 7610.
Holinger, E.P., et al. 1999. J. Biol. Chem. 274, 13298.
Sui, D., and Wilson, J.E. 1997. Arch. Biochem. Biophys. 345, 111.
Product Information
ATP CompetitiveN
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₈₈H₂₉₁N₅₃O₄₀S₂
Chemical formulaC₁₈₈H₂₉₁N₅₃O₄₀S₂
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetDetach and translocate HXK2 from mitochondria to the cytosol
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Met-Ile-Ala-Ser-His-Leu-Leu-Ala-Tyr-Phe-Phe-Thr-Glu-Leu-Asn-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
376816-1MGCN 04055977212976

Documentation

Hexokinase II VDAC Binding Domain Peptide, Cell-Permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Hexokinase II VDAC Binding Domain Peptide, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
376816

References

Reference overview
Pastorino, J.G., et al. 2002. J. Biol. Chem. 277, 7610.
Holinger, E.P., et al. 1999. J. Biol. Chem. 274, 13298.
Sui, D., and Wilson, J.E. 1997. Arch. Biochem. Biophys. 345, 111.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-July-2008 RFH
SynonymsHXK2VBD-cpm, H-RQIKIWFQNRRMKWKK-MIASHLLAYFFTELN-NH₂
DescriptionA cell-permeable peptide analog of Hexokinase II VDAC binding domain (HXK2VBD) peptide (Cat. No. 376815). The internalization sequence of the Antennapedia homeoprotein is fused to the N-terminal methionine of the HXK2VBD peptide. Has been shown to cause the complete release of hexokinase II (HXK2) from the mitochondria and subsequent translocation to the cytosol in HeLa cells (100% release at 100 µM). Does not induce Bax translocation or cytochrome c release. Also shown to markedly sensitize cells to Bax-induced cytochrome c release. Also increases the sensitivity of cells to apoptosis in combination with the Bax-dependent apoptosis inducer, Indomethicin (Cat. No. 405268).
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₈₈H₂₉₁N₅₃O₄₀S₂
Peptide SequenceH-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Met-Ile-Ala-Ser-His-Leu-Leu-Ala-Tyr-Phe-Phe-Thr-Glu-Leu-Asn-NH₂
Purity≥95% by HPLC
SolubilityH₂O (1 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesPastorino, J.G., et al. 2002. J. Biol. Chem. 277, 7610.
Holinger, E.P., et al. 1999. J. Biol. Chem. 274, 13298.
Sui, D., and Wilson, J.E. 1997. Arch. Biochem. Biophys. 345, 111.