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324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

324708
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Overview

Products

Catalogue NumberPackaging Qty/Pack
324708-2UGCN Plastic ampoule 2 μg
Description
OverviewNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalogue Number324708
Brand Family Calbiochem®
References
ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.
Product Information
CAS number9001-92-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
EC number3.4.24.33
FormWhite lyophilized solid
FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥20,000 units/mg protein
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Global Trade Item Number
Catalogue Number GTIN
324708-2UGCN 04055977215939