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345811 Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
345811
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
345811-50ULCN Plastic ampoule 50 ul
Description
OverviewRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Catalogue Number345811
Brand Family Calbiochem®
References
ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
Scudder, P., et al. 1983. Biochem. J. 213, 485.
Product Information
Activity≥14 U/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
EC number3.2.1.103
FormLiquid
FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥140 U/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
345811-50ULCN 04055977214970

Documentation

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli Certificates of Analysis

TitleLot Number
345811

References

Reference overview
Murata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
Scudder, P., et al. 1983. Biochem. J. 213, 485.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2007 JSW
DescriptionRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
FormLiquid
FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
Recommended reaction conditions
Activity Assay
Required Reagents • 5X Reaction Buffer: 250 mM sodium phosphate, pH 5.8 Assay For Glycoproteins 1. Add up to 100 µg glycoprotein to a clean tube. 2. Add 4 µl 5X Reaction Buffer. 3. Add H2O to a total volume of 19 µl. 4. Add 1 µl endo-β-galactosidase. 5. Incubate at 37°C for 2 h. For Oligosaccharides Follow the assay as outlined above in steps 1-4. Incubate at 37°C for several h to several days, depending on the substrate.
EC number3.2.1.103
Specific activity≥140 U/mg protein
Activity≥14 U/ml
Unit definitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
Toxicity Standard Handling
ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
Scudder, P., et al. 1983. Biochem. J. 213, 485.