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262015 DNA Base Excision Repair Pathway Inhibitor - CAS 6960-45-8 - Calbiochem

The DNA Base Excision Repair Pathway Inhibitor, also referenced under CAS 6960-45-8, controls the biological activity of APE1 (human apurinic/apyrimidinic endonuclease.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 7-Nitroindole-2-carboxylic acid, CRT0044876

Primary Target: APE 1
262015
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
6960-45-8C₉H₆N₂O₄

Products

Catalogue NumberPackaging Qty/Pack
262015-25MGCN Plastic ampoule 25 mg
Description
OverviewA cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC50 = ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H2O2 in HeLa, HT1080, and MDA-MB-231 cells.
Catalogue Number262015
Brand Family Calbiochem®
Synonyms7-Nitroindole-2-carboxylic acid, CRT0044876
References
ReferencesMadhusudan, S., et al. 2005. Nucl. Acids Res. 33, 4711.
Product Information
CAS number6960-45-8
ATP CompetitiveN
FormTan solid
Hill FormulaC₉H₆N₂O₄
Chemical formulaC₉H₆N₂O₄
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetAPE 1
Primary Target IC<sub>50</sub>~3 µM against APE1 (human apurinic/apyrimidinic endonuclease
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
262015-25MGCN 04055977216974

Documentation

DNA Base Excision Repair Pathway Inhibitor - CAS 6960-45-8 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

DNA Base Excision Repair Pathway Inhibitor - CAS 6960-45-8 - Calbiochem Certificates of Analysis

TitleLot Number
262015

References

Reference overview
Madhusudan, S., et al. 2005. Nucl. Acids Res. 33, 4711.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2018 JSW
Synonyms7-Nitroindole-2-carboxylic acid, CRT0044876
DescriptionA cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC50 ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H2O2 in HeLa, HT1080, and MDA-MB-231 cells.
FormTan solid
Intert gas (Yes/No) Packaged under inert gas
CAS number6960-45-8
Chemical formulaC₉H₆N₂O₄
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Irritant
ReferencesMadhusudan, S., et al. 2005. Nucl. Acids Res. 33, 4711.