Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

233526 Coagulation Factor Xa, Human Plasma

Coagulation Factor Xa, Human Plasma, CAS 9002-05-5, is a native coagulation factor Xa prepared from Factor X by activation with Russel's viper venom. The venom is removed after activation.

Coagulation Factor Xa, Human Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
233526
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
233526-10UCN Plastic ampoule 10 u
Description
OverviewNative coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. The venom is removed after activation. During coagulation, in the presence of Ca2+ and membrane phospholipids, a complex of Factor Xa and Factor Va (the prothrombinase complex) activates prothrombin to thrombin. Inhibitors include AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and Soybean Trypsin Inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.
Catalogue Number233526
Brand Family Calbiochem®
References
ReferencesHeeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2728.
Lindhout, T., et al. 1994. Biochem. J. 297, 131.
Morishita, H., et al. 1994. Thrombosis Res. 73, 193.
Lupu, C., et al. 1993. Thrombosis Haemostasis 70, 579.
Jordan, S.P., et al. 1992. Biochemistry 31, 5374.
Schoen, P., et al. 1992. Br. J. Haemotol. 81, 255.
Tracy, P.B., et al. 1992. Methods Enzymol. 215, 329.
Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem. 226, 11317.
Tans, G., et al. 1991. J. Biol. Chem. 266, 21864.
Owen, W.G., et al. 1974. J. Biol. Chem. 249, 594.
Product Information
CAS number9002-05-5
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol CHROMOZYM<sup>®</sup> X substrate per min at 25°C.
EC number3.4.21.6
FormLyophilized
FormulationLyophilized from 700 mM NaCl, 20 mM Tris-HCl, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purityhomogeneous by SDS-PAGE
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥170 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
233526-10UCN 04055977217889

Documentation

Coagulation Factor Xa, Human Plasma SDS

Title

Safety Data Sheet (SDS) 

Coagulation Factor Xa, Human Plasma Certificates of Analysis

TitleLot Number
233526

References

Reference overview
Heeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2728.
Lindhout, T., et al. 1994. Biochem. J. 297, 131.
Morishita, H., et al. 1994. Thrombosis Res. 73, 193.
Lupu, C., et al. 1993. Thrombosis Haemostasis 70, 579.
Jordan, S.P., et al. 1992. Biochemistry 31, 5374.
Schoen, P., et al. 1992. Br. J. Haemotol. 81, 255.
Tracy, P.B., et al. 1992. Methods Enzymol. 215, 329.
Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem. 226, 11317.
Tans, G., et al. 1991. J. Biol. Chem. 266, 21864.
Owen, W.G., et al. 1974. J. Biol. Chem. 249, 594.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-March-2009 JSW
DescriptionNative coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. Hydrolyzes peptide and ester bonds at the carboxyl group of arginine. Specifically catalyzes the activation of prothrombin to thrombin. Prothrombin activation is accelerated by Factor Va, phospholipids, and Ca2+. Factor Xa was prepared from homogeneous Factor X, using Russels' viper venom (RVV), which was removed after activation. Inhibited by AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and soybean trypsin inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.
FormLyophilized
FormulationLyophilized from 700 mM NaCl, 20 mM Tris-HCl, pH 7.4.
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number9002-05-5
EC number3.4.21.6
Purityhomogeneous by SDS-PAGE
Specific activity≥170 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol CHROMOZYM® X substrate per min at 25°C.
SolubilityH₂O.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C.
Toxicity Standard Handling
ReferencesHeeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2728.
Lindhout, T., et al. 1994. Biochem. J. 297, 131.
Morishita, H., et al. 1994. Thrombosis Res. 73, 193.
Lupu, C., et al. 1993. Thrombosis Haemostasis 70, 579.
Jordan, S.P., et al. 1992. Biochemistry 31, 5374.
Schoen, P., et al. 1992. Br. J. Haemotol. 81, 255.
Tracy, P.B., et al. 1992. Methods Enzymol. 215, 329.
Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem. 226, 11317.
Tans, G., et al. 1991. J. Biol. Chem. 266, 21864.
Owen, W.G., et al. 1974. J. Biol. Chem. 249, 594.