Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

HTS129M ChemiScreen™ D5 Dopamine Receptor Membrane Preparation

Human D5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P21918 Entrez Gene Number: NM_000798.3
HTS129M
200 units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1D5
Description
Catalogue NumberHTS129M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiScreen™ D5 Dopamine Receptor Membrane Preparation
OverviewFull-length human DRD5 cDNA encoding D5
Background InformationDopamine is a catecholamine neurotransmitter that functions in the CNS to control locomotor, cognitive, emotional and neurendocrine processes, and in the periphery to modulate cardiovascular, renal and gastrointestinal processes. The biological activities of dopamine are mediated by a family of five GPCRs. The D1 and D5 subtypes couple to Gs to increase intracellular cAMP, whereas the D2, D3 and D4 subtypes couple to Gi to reduce cAMP (Missale et al., 1998). The hypertensive phenotype of mice with a targeted deletion of D5 indicates that D5 regulates central control of sympathetic vascular tone (Hollon et al., 2002). In addition, D5 modulates locomotion and corticostriatal long-term depression (Centonze et al., 2003). Chemicon's cloned human D5-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant D5 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between D5 and its ligands. The membrane preparations exhibit a Kd of 2.25 nM for [3H]-SCH 23390. With 0.5 nM [3H]-SCH 23390, 10 µg/well D5 Membrane Prep yields greater than 3-fold signal-to-background ratio.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to 1 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80%deg;C.
Quality LevelMQ100
Applications
ApplicationHuman D5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay and GTPγS binding.
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2.
Gene Symbol
  • DRD5
  • DRD1B
  • DBDR
  • DRD1L2
  • D
  • MGC10601
GPCR ClassA
Host CellsChem-1
Protein TargetD5
Target Sub-FamilyDopamine
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P21918 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 477 amino acids; 52951 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Neuron-specific, localized primarily within limbic regions of the brain.
DISEASE: SwissProt: P21918 # Defects in DRD5 may be a cause of schizophrenia, but no proof has yet been found. & Defects in DRD5 are a cause of blepharospasm [MIM:606798]. It is a primary focal dystonia affecting the orbicularis oculi muscles, usually beginning in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. In severe cases, this can lead to functional blindness.
SIMILARITY: SwissProt: P21918 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H]-SCH 23390. (Perkin Elmer#:NET-930 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H labeled SCH 23390 at 0.5 nM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Signal:background and specific binding values obtained in a competition binding assay with varying amounts of D5 membrane prep:
Product Usage Statements
10 µg/well
Signal:Background 4.06
Specific Binding (cpm) 1952


SPECIFICATIONS: 1 unit = 10 µg
Bmax for [3H] SCH 23390 binding: 11.42 pmol/mg protein;
Kd for [3H] SCH 23390 binding: ~ 2.25 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS129M 04053252731112

Documentation

ChemiScreen™ D5 Dopamine Receptor Membrane Preparation SDS

Title

Safety Data Sheet (SDS) 

ChemiScreen™ D5 Dopamine Receptor Membrane Preparation Certificates of Analysis

TitleLot Number
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN D5 DOPAMINE RECEPTOR - 2278666 2278666

References

Reference overviewPub Med ID
Distinct roles of D1 and D5 dopamine receptors in motor activity and striatal synaptic plasticity
Centonze, Diego, et al
J Neurosci, 23:8506-12 (2003)  2003

13679419 13679419
Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive
Hollon, Tom R, et al
J Neurosci, 22:10801-10 (2002)  2002

12486173 12486173
Dopamine receptors: from structure to function
Missale, C, et al
Physiol Rev, 78:189-225 (1998)  1998

9457173 9457173

Related Products & Applications

Product Families

ChemiSCREEN™ GPCR Stable Cell Lines, Membranes Preparation and Ready-to-Assay™ Frozen Cells

Millipore offers a large selection of robust and reliable G-protein coupled receptor products, including Stable Cell Lines, Membrane Preparations, and Frozen Cells. See below for GPCR research tools.
Learn More >>

Related Products By: Brand Facete

ChemiScreen™

Categories

Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations