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264150 Caspase-3 Substrate IV, Fluorogenic - CAS 1177935-21-5 - Calbiochem

Fluorogenic caspase-3 substrate.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Z-DEVD-AFC

264150
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
264150-1MGCN Plastic ampoule 1 mg
Description
OverviewFluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.
Catalogue Number264150
Brand Family Calbiochem®
SynonymsZ-DEVD-AFC
References
ReferencesKeramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science 275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem. 272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol. 6, 897.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Product Information
CAS number1177935-21-5
ATP CompetitiveN
FormWhite or off-white solid
Hill FormulaC₃₆H₃₈F₃N₅O₁₄
Chemical formulaC₃₆H₃₈F₃N₅O₁₄
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetFluorogenic caspase-3 substrate.
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceZ-Asp-Glu-Val-Asp-AFC
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
264150-1MGCN 04055977198409

Documentation

Caspase-3 Substrate IV, Fluorogenic - CAS 1177935-21-5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Caspase-3 Substrate IV, Fluorogenic - CAS 1177935-21-5 - Calbiochem Certificates of Analysis

TitleLot Number
264150

References

Reference overview
Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science 275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem. 272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol. 6, 897.
Nicholson, D.W., et al. 1995. Nature 376, 37.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2011 RFH
SynonymsZ-DEVD-AFC
DescriptionFluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.
FormWhite or off-white solid
CAS number1177935-21-5
Chemical formulaC₃₆H₃₈F₃N₅O₁₄
Peptide SequenceZ-Asp-Glu-Val-Asp-AFC
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (10 mg/ml)
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.
Toxicity Standard Handling
ReferencesKeramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science 275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem. 272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol. 6, 897.
Nicholson, D.W., et al. 1995. Nature 376, 37.