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208771 Calpain Substrate III, Fluorogenic - Calbiochem

A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (DABCYL)-TPLK~SPPPSPR-(EDANS)

208771
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Overview

Products

Catalogue NumberPackaging Qty/Pack
208771-1MGCN Plastic ampoule 1 mg
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Description
OverviewA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
Catalogue Number208771
Brand Family Calbiochem®
Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
References
ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
Product Information
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₈₀H₁₁₄N₂₀O₁₈S
Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
ReversibleN
Quality LevelMQ100
Biological Information
Primary TargetSubstrate sequence that is optimized for calpain-1 and -2
Primary Target IC<sub>50</sub>kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Catalogue Number GTIN
208771-1MGCN 04055977202854