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208748 Calpain-1 Substrate, Fluorogenic - Calbiochem

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH

208748
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
208748-2MGCN Plastic ampoule 2 mg
Description
OverviewAn internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.
Catalogue Number208748
Brand Family Calbiochem®
Synonymsμ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
References
ReferencesMittoo, S., et al. 2003. Anal. Biochem. 319, 234.
Product Information
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₇₉H₉₅N₁₃O₁₉S₂
Chemical formulaC₇₉H₉₅N₁₃O₁₉S₂
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetA sensitive and specific substrate for calpain-1
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceH-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208748-2MGCN 04055977202830

Documentation

Calpain-1 Substrate, Fluorogenic - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Calpain-1 Substrate, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
208748

References

Reference overview
Mittoo, S., et al. 2003. Anal. Biochem. 319, 234.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-May-2008 RFH
Synonymsμ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
DescriptionAn internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions50 mM Tris-HCl, 50 mM NaCl, 5 mM β-mercaptoethanol, 5 mM CaCl2, 1 mM EDTA, 1 mM EGTA, and 1 µM substrate, pH 7.5.
Chemical formulaC₇₉H₉₅N₁₃O₁₉S₂
Peptide SequenceH-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
Purity≥95% by HPLC
SolubilityDMSO (1 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesMittoo, S., et al. 2003. Anal. Biochem. 319, 234.