Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

206700 Calcein-AM - CAS 148504-34-1 - Calbiochem

A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein.

Calcein-AM - CAS 148504-34-1 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Calcein-acetoxymethyl Ester, Diacetate, CAL-AM

206700
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
148504-34-1C₄₆H₄₆N₂O₂₃

Products

Catalogue NumberPackaging Qty/Pack
206700-1MGCN Plastic ampoule 1 mg
Description
OverviewA cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein. Can also be used for imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis, and multidrug resistance (MDR).
Catalogue Number206700
Brand Family Calbiochem®
SynonymsCalcein-acetoxymethyl Ester, Diacetate, CAL-AM
References
ReferencesDhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
Feller, N., et al. 1995. Br. J. Cancer 72, 543.
Feller, N., et al. 1995. FEBS Lett. 368, 385.
Product Information
CAS number148504-34-1
ATP CompetitiveN
FormOff-white solid
Hill FormulaC₄₆H₄₆N₂O₂₃
Chemical formulaC₄₆H₄₆N₂O₂₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetImaging mitochondrial permeability pore transition
Purity≥90% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
206700-1MGCN 04055977203172

Documentation

Calcein-AM - CAS 148504-34-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Calcein-AM - CAS 148504-34-1 - Calbiochem Certificates of Analysis

TitleLot Number
206700

References

Reference overview
Dhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
Feller, N., et al. 1995. Br. J. Cancer 72, 543.
Feller, N., et al. 1995. FEBS Lett. 368, 385.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2008 JSW
SynonymsCalcein-acetoxymethyl Ester, Diacetate, CAL-AM
DescriptionA cell-permeable, nonfluorescent, and hydrophobic compound, which is rapidly hydrolyzed by cytoplasmic esterases inside the cell, releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein (excitation maximum: 494 nm; emission maximum: 517 nm). A cytosolic fluorophore that can also be used imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, and BCECF and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis and multidrug resistance (MDR).
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
CAS number148504-34-1
Chemical formulaC₄₆H₄₆N₂O₂₃
Structure formulaStructure formula
Purity≥90% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Toxicity Standard Handling
ReferencesDhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
Feller, N., et al. 1995. Br. J. Cancer 72, 543.
Feller, N., et al. 1995. FEBS Lett. 368, 385.