Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

100079 Bolton Broth Selective

100079
Purchase on Sigma-Aldrich

Overview

Replacement Information
Replacement InformationIn North America 1000790010 replaces and is identical to 1.00079.0017

Products

Catalogue NumberPackaging Qty/Pack
1.00079.0010 Fibre case 10 x 1 vial
Description
Catalogue Number100079
Replaces1.00079.0017
References
Product Information
HS Code3821 00 00
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H317: May cause an allergic skin reaction.
H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H361d: Suspected of damaging the unborn child.
H373: May cause damage to organs through prolonged or repeated exposure.
Precautionary Statement(s)P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P304 + P340 + P312: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor if you feel unwell.
P308 + P313: IF exposed or concerned: Get medical advice/ attention.
Signal WordDanger
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +8°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDKein Gefahrgut
Declaration (transport by air) IATA-DGRNo Dangerous Good
Declaration (transport by sea) IMDG-CodeNo Dangerous Good
Supplemental Information
Specifications
Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004)

10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005)10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156)10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023)≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004)

> 10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005)> 10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156)> 10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013)

no growth
- Escherichia coli ATCC 8739 (WDCM 00012)no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023)no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004)

greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005)greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156)greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic
Global Trade Item Number
Catalogue Number GTIN
1.00079.0010 04027269168544

Related Products & Applications

Accessories (Primary)

Catalogue Number Description
100068 BOLTON broth (base)

Accessories (Secondary)

Catalogue Number Description
100068 BOLTON broth (base)
104143 Singlepath® Campylobacter

Categories

Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > Campylobacter
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Product > Dehydrated and Ready-to-use Culture Media
Industrial Microbiology > Culture Media > Raw Materials & Supplements > Supplements for Culture Media