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SCT038 BioTracker Orange OH and HClO Dye

Live cell imaging probe to detect hydroxy radical (OH) or hypochlorous acid (HClO) in live cells.

SCT038
5 x 100 nmol  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT038
DescriptionBioTracker Orange OH and HClO Dye
OverviewReactive oxygen species (ROS) are important mediators in a variety of biological and pathological events. For example, ROS generated in mitochondria play important roles in the modulation of signal transduction cascades and transcription factors. However, oxidative stress due to excessive generation of ROS is implicated in many human diseases, including acute and chronic inflammatory processes, ischemic stroke, diabetes, sepsis, ischemia-reperfusion injury, atherosclerosis, and neurodegenerative disorders.

BioTracker Orange OH and HClO Dye is an orange fluorescent probe to detect hydroxy radical (・OH) or hypochlorous acid (HClO) in live-cell imaging. It can detect -OH and HClO among other reactive oxygen species (O2-, H2O2, NO, ONOO-). Its nearly red fluorescence spectrum allows multicolor imaging with green (ex. GFP, FITC) and blue (ex. Hoechst 33342) fluorophores. Because of its positive charge, OxiORANGE™ tends to localize within mitochondria. It has high photostability and is suitable for time-lapse imaging of intracellular hROS generation.

Spectral Properties
Absorbance (max): 553m
Emission (max): 577nm

References
Koide Y et al. Design and Synthesis of Fluorescent Probes for Selective Detection of Highly Reactive Oxygen Species in Mitochondria of Living Cells. J. Am. Chem Soc. 2007, 129, 34, 10324-10325.
Alternate Names
  • OxiORANGE™
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging probe to detect hydroxy radical (OH) or hypochlorous acid (HClO) in live cells.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging. Red/Orange fluorescence spectrum.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 95% (LC)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker Orange OH and HClO Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size5 x 100 nmol
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT038 04061841588245