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SCT086 BioTracker MitoBlue Live Cell Dye

Live cell imaging dye that stains functional mitochondria and tracks the mitochondrial degradation process and transport to lysosomes.

SCT086
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT086
DescriptionBioTracker MitoBlue Live Cell Dye
OverviewBioTracker MitoBlue Live Cell Dye is a synthetic bisbenzamidine derivative that selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching in both live and fixed cells (1). It is a unique mitochondrial dye in that after initially staining mitochondria, the dye is transported to lysosomes in a time-dependent manner. This distinct staining pattern allows for monitoring and tracking the mitochondrial recycling mechanism in living cells (2). MitoBlue has been shown to be suitable for two-photon microscopy using a 740 nm two-photon excitation (TPE) wavelength and emission channel at 480 nm (2).

Spectral Properties
Absorbance: 329nm
Emission: 420nm

Reference:
1. Sánchez, Mateo I., et al. "MitoBlue: a nontoxic and photostable blue-emitting dye that selectively labels functional mitochondria." ACS chemical biology 9.12 (2014): 2742-2747.
2. Sánchez, Mateo I., et al. "MitoBlue as a tool to analyze the mitochondria-lysosome communication." Scientific reports 10.1 (2020): 1-12.
Alternate Names
  • Live cell imaging probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized. Yellow solid.
Applications
ApplicationLive cell imaging dye that stains functional mitochondria and tracks the mitochondrial degradation process and transport to lysosomes.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Molecular Weight866.61
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity ≥ 97% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C, desiccate and protect from light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT086 04065266515664