506048 Sigma-AldrichBeclin-1 Activator I, TAT-Beclin-1 - Calbiochem
Beclin-1 Activator I, TAT-Beclin-1, is a cell-permeable Atg6/Beclin1-derived autophagy-inducing peptide. Activates Beclin1 by competing against its negative regulator GAPR-1/GLIPR2.
More>> Beclin-1 Activator I, TAT-Beclin-1, is a cell-permeable Atg6/Beclin1-derived autophagy-inducing peptide. Activates Beclin1 by competing against its negative regulator GAPR-1/GLIPR2. Less<<Synonyms: H₂N-YGRKKRRQRRR-GG-TNVFNATFEIWHDGEFGT-CO₂H, Atg6 Activator I, Beclin1-GAPR-1 Interaction Blocker I, Vps30 Activator I, Autophagy Inducer IV
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Overview
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Key Spec Table
Empirical Formula |
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C₁₆₄H₂₅₁N₅₇O₄₅ |
Products
Catalogue Number | Packaging | Qty/Pack | |
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5.06048.0001.1753 | Glass bottle | 10 mg |
Description | |
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Overview | A cell-permeable Atg6/Beclin1-derived autophagy-inducing peptide (Minimum effective conc. ≤10 µM for 3 h) that effectively activates cellular Beclin1 by competing against Beclin1 for binding to its negative regulator GAPR-1/GLIPR2 on the Golgi surface. Shown to induce autosis, autophagy-dependent death, in cells of human, rat, and murine origin in a dose- and time-dependent manner (Minimum effective conc. >5 µM for 5 h in HeLa cultures), blockable by autophagy inhibitor 3-MA (10 mM, Cat. no.189490; [T-B] = 20 µM) or via Na+/K+-ATPase inhibition, but insensitive to Z-VAD-FMK (Cat. no. 219007) or Nec-1 (Cat. nos. 480065 & 505224) treatment. Non-cytotoxic autophagy induction with lower doses of Tat-Beclin1 and shorter exposure times is therapeutically beneficial in blocking RNA viral replications both in cultures (4 h 10 µM peptide treatment 4 h post viral infection in HeLa cultures; 0.5-5 µM peptide pre-treatment 24 h before HIV infection in human MDM cultures) in vitro and in CHIKV-infected neonatal mice (15 mg/kg/day i.p.) in vivo. While Tat-Beclin1 bioavailability is limited to the peripheral tissues in mice, a peptide based on Tat-Beclin1 retro-inverso sequence (Cat. no. 506416) shows efficacy in a murine model of neonatal WNV CNS infection. Note: increased drug potency is reported in acidified (pH 7.0) serum-free cultures. |
Catalogue Number | 506048 |
Brand Family | Calbiochem® |
Synonyms | H₂N-YGRKKRRQRRR-GG-TNVFNATFEIWHDGEFGT-CO₂H, Atg6 Activator I, Beclin1-GAPR-1 Interaction Blocker I, Vps30 Activator I, Autophagy Inducer IV |
References | |
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References | Liu, Y., et al. 2013. Proc. Natl. Acad. Sci. USA 110, 20634. Shoji-Kawata, S., et al. 2013. Nature. 494, 201. |
Product Information | |
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Declaration | Sold under license of US Patent 8,722,628. |
Form | White powder |
Formulation | Supplied as a trifluoroacetate salt. |
Hill Formula | C₁₆₄H₂₅₁N₅₇O₄₅ |
Chemical formula | C₁₆₄H₂₅₁N₅₇O₄₅ |
Hygroscopic | Hygroscopic |
Reversible | Y |
Quality Level | MQ100 |
Biological Information | |
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Primary Target | GAPR-1/GLIPR2 |
Purity | ≥97% by HPLC |
Physicochemical Information | |
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Cell permeable | Y |
Peptide Sequence | H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg~Gly-Gly~Thr-Asn-Val-Phe-Asn-Ala-Thr-Phe-Glu-Ile-Trp-His-Asp-Gly-Glu-Phe-Gly-Thr-OH |
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Packaged under inert gas | Packaged under inert gas |
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Specifications |
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Global Trade Item Number | |
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Catalogue Number | GTIN |
5.06048.0001.1753 | 04055977262704 |
Documentation
Beclin-1 Activator I, TAT-Beclin-1 - Calbiochem SDS
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References
Reference overview |
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Liu, Y., et al. 2013. Proc. Natl. Acad. Sci. USA 110, 20634. Shoji-Kawata, S., et al. 2013. Nature. 494, 201. |