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06-597-25UG Anti-phospho-Histone H1

Anti-phospho-Histone H1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of phospho-Histone H1 & has been validated in ICC & WB.

Anti-phospho-Histone H1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H1C, MGC3992, H1F2, H1.2 UniProt Number: P16403
06-597-25UG
25 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HICC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue Number06-597-25UG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Histone H1
Background InformationHistone H1 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H1 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

The N-terminal tail of histone H1 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Product Information
FormatPurified
Control
  • Colcemid treated HeLa cell lysate
PresentationImmunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, PH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Histone H1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of phospho-Histone H1 & has been validated in ICC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Biological Information
ImmunogenHyper-phosphorylated isoform of tetrahymena Histone H1 purified by cation-exchange HPLC
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes phospho-Histone H1, Mr 32 kDa. Additional proteins were also detected, Mr 66 and 100 kDa.
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Gene Symbol
  • HIST1H1C
  • MGC3992
  • H1F2
  • H1.2
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16403 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
SIZE: 213 amino acids; 21365 Da
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: P16403 ## Belongs to the histone H1/H5 family.
Molecular Weight32 kDa
Product Usage Statements
Quality AssuranceImmunoblot Analysis: 0.5-2 μg/mL detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells (Catalog # 17-306).

Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol/5% glacial acetic acid (v/v).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size25 μg
Global Trade Item Number
Catalogue Number GTIN
06-597-25UG 04054839350443