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MABT1443-25UL Anti-phospho-Connexin 43 (Ser368) Antibody, clone 2C6

Anti-phospho-Connexin 43 (Ser368), clone 2C6, Cat. No. MABT1443, is a mouse monoclonal antibody that detects Connexin-43 phosphorylated on serine 368 and is tested for use in Western Blotting.

MABT1443-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1443-25UL
DescriptionAnti-phospho-Connexin 43 (Ser368) Antibody, clone 2C6
Alternate Names
  • Gap junction alpha-1 protein
  • Connexin-43
  • Cx43
  • Gap junction 43 kDa heart protein
Background InformationGap junction alpha-1 protein (UniProt: P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as GJAL) gene (Gene ID: 2697) in human. Cx43 is a member of the gap junction family, which is composed of intercellular channels allowing direct cell-to-cell communication between neighboring cells via the exchange of small ions and metabolites. It is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is one of the most widely studied gap junction proteins that regulates N-cadherin expression during collective cell migration. It also acts as a negative regulator of bladder capacity by enhancing intercellular electrical and chemical transmission and thereby exsensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. It is also expressed in gap junctions of the cardiac ventricle and plays an essential role in gap junction communication in the ventricles. Cx43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Cx43 is phosphorylated at Ser-368 by PKCg (PRKCG) and this phosphorylation is reported to induce disassembly of gap junction plaques and inhibition of gap junction activity and triggers its internalization into small vesicles leading to proteasome-mediated degradation. (Ref.: Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596; Kotini, M., et al. (2018). Nature Commun. 9; article 3846).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-phospho-Connexin 43 (Ser368), clone 2C6, Cat. No. MABT1443, is a mouse monoclonal antibody that detects Connexin-43 phosphorylated on serine 368 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis (WB): A representative lot of this antibody detected phospho-Connexin-43 (Ser368) in NRK cells. ( Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596).


Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 368 from the C-terminal, cytoplasmic domain of human Connexin 43.
EpitopeC-terminal
Clone2C6
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 2C6 is a mouse monoclonal antibody that detects Connexin 43 phosphorylated on serine 368.
IsotypeIgG2aκ
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human, Bovine, Canine, Feline, Monkey, Porcine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GJA1
Modifications
  • Phosphorylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~42 kDa observed; 43.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NRK cell lysate.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Connexin 43 phosphorylated on serine 368 in lysates from untreated NRK cells and in NRK cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA; 25 nM; 40 min).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1443-25UL 04065267060347