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07-303-25UG Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal)

Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody is an antibody against phospho-Acetyl CoA Carboxylase (Ser79) for use in WB.

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Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: ACACA, ACC-alpha, ACCA, ACC, ACC1, ACAC UniProt Number: Q13085
07-303-25UG
25 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Ca, Ch, BWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-303-25UG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal)
Alternate Names
  • acetyl-CoA carboxylase 1
  • acetyl-CoA carboxylase-alpha
  • acetyl-Coenzyme A carboxylase alpha
Background InformationAcetyl CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by the reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones, glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors of the cell, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl CoA carboxylase is thus enhanced. Acetyl CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
References
Product Information
FormatPurified
PresentationProtein A purified in buffer containing in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% NaN3.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody is an antibody against phospho-Acetyl CoA Carboxylase (Ser79) for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH conjugated synthetic peptide (C-HM RSSM[pS]GLHLVK) corresponding to amino acid 73-85 of rat Acetyl CoA Carboxylase.
EpitopeN-terminal
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes Acetyl CoA Carboxylase phosphorylated at Serine 79, MW 257 kDa.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Canine
  • Chicken
  • Bovine
Species Reactivity NoteHuman. Expected to react with Cow, Dog, Chicken, Rat and Mouse based on immunogen sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAcetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Gene Symbol
  • ACACA
  • ACC-alpha
  • ACCA
  • ACC
  • ACC1
  • ACAC
Modifications
  • Phosphorylation
Purification MethodProtein A chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain.
Molecular Weight257 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on Lambda phosphatase-treated and untreated A375 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected endogenous phospho-Acetyl CoA Carboxylase (Ser79) in lysate from Lambda phosphatase-treated and untreated A375 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-303-25UG 04054839337550

Documentation

Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) Certificates of Analysis

TitleLot Number
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) 3079066
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) - 3172382 3172382
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) - 3313743 3313743
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) - 3445821 3445821
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) - 3546510 3546510
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) - 3780270 3780270
Anti-phospho-Acetyl CoA Carboxylase (Ser79) (rabbit polyclonal) Polyclonal Antibody 2987657