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OP23 Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP23
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP23-100UGCN Plastic ampoule 100 μg
OP23-200UGCN Plastic ampoule 200 μg
Description
OverviewRecognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
Catalogue NumberOP23
Brand Family Calbiochem®
References
ReferencesRodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Positive controlNormal skin tissue or ras 1 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (5 µg/ml)
Immunoblotting (10 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Application CommentsFor immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant p21 Ras
Epitopewithin amino acids 54-188
CloneF235-1.7.1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP23-100UGCN 04055977208962
OP23-200UGCN 04055977208986

Documentation

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) Certificates of Analysis

TitleLot Number
OP23

References

Reference overview
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationFrozen Sections (5 µg/ml)
Immunoblotting (10 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-Ras are the homologues of v-H- and v-K-Ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and their relevance to human disease diagnosis and prognosis is still emerging.
HostMouse
Immunogenrecombinant p21 Ras
Epitopewithin amino acids 54-188
CloneF235-1.7.1
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlNormal skin tissue or ras 1 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesRodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.