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OP16 Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes ~185 kDa c-ErbB2 in overexpressing B104-1-1 cells. It is validated for WB, IP, ICC, frozen sections, & growth inhibition assay.

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2

OP16
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP16-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
Catalogue NumberOP16
Brand Family Calbiochem®
SynonymsAnti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
Application Data
Detection of rat c-neu by immunocytochemistry. Sample: B104-1-1 cells overexpressing c-neu. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) (Cat. No. OP16) (1.4 µg/ml). Detection: fluorescence.
References
ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
DiFiore, P.P., et al. 1987. Science 237, 178.
Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
Slamon, D.J., et al. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative controlFisher rat embryo cells
Positive controlB104-1-1 cells
Preservative≤0.1% sodium azide
Quality LevelMQ200
Applications
Application ReferencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Not for Neutralization Studies Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Not Neutralization Studies
Application NotesFrozen Sections (5 µg/ml, see application references)
Immunoblotting (see application references)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (1 µg/ml, see application references)
Neutralization Studies (not recommended, see application references)
Application CommentsStains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
ImmunogenHuman
EpitopeWithin the extracellular domain
Clone7.16.4
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP16-100UGCN 04055977208801

Documentation

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Certificates of Analysis

TitleLot Number
OP16

References

Reference overview
Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
DiFiore, P.P., et al. 1987. Science 237, 178.
Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
Slamon, D.J., et al. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

Citations

Title
  • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    SynonymsAnti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
    ApplicationFrozen Sections (5 µg/ml, see application references)
    Immunoblotting (see application references)
    Immunocytochemistry (5 µg/ml)
    Immunoprecipitation (1 µg/ml, see application references)
    Neutralization Studies (not recommended, see application references)
    Application Data
    Detection of rat c-neu by immunocytochemistry. Sample: B104-1-1 cells overexpressing c-neu. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) (Cat. No. OP16) (1.4 µg/ml). Detection: fluorescence.
    DescriptionPurified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein.
    BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
    HostMouse
    Immunogen speciesHuman
    ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
    EpitopeWithin the extracellular domain
    Clone7.16.4
    IsotypeIgG2a
    Specieshuman, rat
    Positive controlB104-1-1 cells
    Negative controlFisher rat embryo cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsStains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
    Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
    Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
    Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
    DiFiore, P.P., et al. 1987. Science 237, 178.
    Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
    Slamon, D.J., et al. Science 235, 177.
    Varley, J.M., et al. 1987. Oncogene 1, 423.
    Bargmann, C.I., et al. 1986. Nature 319, 226.
    Yamamoto, T., et al. 1986. Nature 319, 230.
    Blick, M., et al. 1984. Blood 64, 1234.
    Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
    Citation
  • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
    		•
    Application referencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Not for Neutralization Studies Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.